| Protein name |
Chymosin B |
| Host compatibility |
Aspergillus niger |
| Yield |
>1 g/L (Dunn-Coleman et al., 1991)
|
| Scale (in specific example) |
Lab scale in flask (Dunn-Coleman et al., 1991) *
|
| Biggest scale product has been develloped in this organism |
Industrial (Chr. Hansen) |
| Regulatory status in Europe |
Production of chymosin is issued as safe for consumption by EFSA, but is not allowed for the use in food yet (Silano et al., 2022)
|
| Regulatory status in other parts of the world |
FDA GRAS approval in the US. Not allowed in Canada
|
| Companies producing this protein in this organism |
Chr. Hansen (part of Novonesis)
|
| Extra/remark |
|
| Publications/references |
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Dunn-Coleman, N. S., Bloebaum, P., Berka, R. M., Bodie, E., Robinson, N., Armstrong, G., Ward, M., Przetak, M., Carter, G. L., LaCost, R., Wilson, L. J., Kodama, K. H., Baliu, E. F., Bower, B., Lamsa, M., & Heinsohn, H. (1991). Commercial Levels of Chymosin Production by Aspergillus. Bio/Technology, 9(10), 976–981. https://doi.org/10.1038/nbt1091-976
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Barros, R. F., De Marco, J. L., Piva, L. C., Coelho, C. M., De Moraes, L. M. P., & Torres, F. A. (2025). A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii. Journal of Dairy Science. https://doi.org/10.3168/jds.2025-26935
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Chitpinityol, S., & Crabbe, M. C. (1998). Chymosin and aspartic proteinases. Food Chemistry, 61(4), 395–418. https://doi.org/10.1016/s0308-8146(97)00090-3
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Hayes, M. G., Oliveira, J. C., McSWEENEY, P. L. H., & Kelly, A. L. (2002). Thermal inactivation of chymosin during cheese manufacture. Journal of Dairy Research, 69(2), 269–279. https://doi.org/10.1017/s0022029902005472
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Vallejo, J., Ageitos, J., Poza, M., & Villa, T. (2012). Short communication: A comparative analysis of recombinant chymosins. Journal of Dairy Science, 95(2), 609–613. https://doi.org/10.3168/jds.2011-4445
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Fährenmark, J., Lagerlund, I., Morenweiser, R., Caussette, M., Piron, E., & Ab, A. (2002, June 27). WO2002050253A1 - A method for the purification of chymosin - Google Patents. Retrieved September 4, 2025, from https://patents.google.com/patent/WO2002050253A1/enI
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